Cane we use split function in loops to add element in the list directly?

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Welcome!

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I do not understand what you want, but no, you cannot use split to add anything to a list:

>>> help([].split)
Traceback (most recent call last):
  File "<stdin>", line 1, in <module>
AttributeError: 'list' object has no attribute 'split'

Here you can find the list methods, to add items there are append, extend and insert.